Device

Part:BBa_K1053110:Design

Designed by: Eri Takagi   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

Pconst.(High)-RNA scaffold-double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

P-RNA_scaffold-DT.GIF

Source

The sequence for “MS2 aptamer-PP7 aptamer” was cloned using BBa_K738002 (ZJU-China 2012).

References

Delebecque, C.J., Lindner, A.B., Silver, P.A. & Aldaye, F.A., “Organization of Intracellular Reactions with Rationally Designed RNA Assemblies” Science 333, 470-474 (2011).